Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit mAb (CABP1426) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-Chk1-S280 Monoclonal Antibody (CABP1426) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using state-of-the-art monoclonal technology, specifically recognizes the phosphorylated form of Chk1 at serine 280, allowing for precise detection and analysis in various experimental settings.Chk1 is a key player in ensuring genome stability and cell survival under stress conditions, making it an attractive target for cancer research and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Chk1-S280 Monoclonal Antibody
SKU:
CABP1426
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC3123
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:200 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
1111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-Chk1 (S280) Rabbit mAb (CABP1426) at 1:500 dilution. 293T cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
