Anti-YKL-40/CHI3L1 Antibody (CAB1723)

Antibody Name:	Anti-YKL-40 / CHI3L1 Antibody
Antibody SKU:	CAB1723
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, HT-29, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YKLV CYYT SWSQ YREG DGSC FPDA LDRF LCTH IIYS FANI SNDH IDTW EWND VTLY GMLN TLKN RNPN LKTL LSVG GWNF GSQR FSKI ASNT QSRR TFIK SVPP FLRT HGFD GLDL AWLY PGRR DKQH FTTL IKEM KAEF IKEA QPGK KQLL LSAA LSAG KVTI DSSY DIAK ISQH LDFI SIMT YDFH GAWR GTTG HHSP LFRG QEDA SPDR FSNT DYA
Gene ID:	1116
Uniprot:	P36222
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Secreted, extracellular space, perinuclear region
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

CHI3L1, ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YKL-40, YKL40, YYL-40, hCGP-39

Background:

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

UniProt Protein Function:	CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted; Cell adhesion Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: extracellular space; proteinaceous extracellular matrix; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm Molecular Function:extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; apoptosis; response to mechanical stimulus; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7
NCBI Summary:	Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
UniProt Code:	P36222
NCBI GenInfo Identifier:	84028186
NCBI Gene ID:	1116
NCBI Accession:	P36222.2
UniProt Secondary Accession:	P36222,P30923, Q8IVA4, Q96HI7, B2R7B0,
UniProt Related Accession:	P36222
Molecular Weight:	42,625 Da
NCBI Full Name:	Chitinase-3-like protein 1
NCBI Synonym Full Names:	chitinase 3-like 1 (cartilage glycoprotein-39)
NCBI Official Symbol:	CHI3L1
NCBI Official Synonym Symbols:	GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
NCBI Protein Information:	chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39
UniProt Protein Name:	Chitinase-3-like protein 1
UniProt Synonym Protein Names:	39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40
Protein Family:	Chitinase-3-like protein
UniProt Gene Name:	CHI3L1
UniProt Entry Name:	CH3L1_HUMAN

	Western blot analysis of extracts of various cell lines, using YKL-40 / CHI3L1 antibody (CAB1723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat spleen using YKL-40 / CHI3L1 antibody (CAB1723) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using YKL-40 / CHI3L1 antibody (CAB1723) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40 / CHI3L1 antibody (CAB1723) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Raw264.7 cells using YKL-40 / CHI3L1 antibody (CAB1723) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-YKL-40 / CHI3L1 Antibody (CAB3166)	Human CHI3L1 (Chitinase-3-like protein 1) ELISA Kit
Anti-CHI3L1 Antibody (CAB11510)	Human GP39 (Glycoprotein 39, Cartilage) CLIA Kit (HUES00036)
	Human GP39 (Glycoprotein 39, Cartilage) ELISA Kit (HUES01324)
	Human Chitinase-3-like protein 1 (CHI3L1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)