Chicken Anti-COL2 (Collagen Type II) Antibody ELISA Kit
The Chicken Anti-COL2 (Collagen Type II) Antibody ELISA Kit from AssayGenie is a cutting-edge tool for precise measurement of Anti-COL2 Antibody levels in chicken serum, plasma, cell culture supernatant and other biological samples. This assay delivers exceptional sensitivity and specificity, ensuring accurate quantification of target antibodies across diverse sample matrices. Anti-COL2 antibodies are autoimmune markers that bind to collagen type II, a major structural component of cartilage and connective tissue. These antibodies are implicated in degenerative joint diseases and arthropathies in poultry, where aberrant immune responses against self-collagen drive inflammatory cartilage destruction and compromise skeletal integrity.
Equipped with this ELISA kit, researchers can delve deep into the intricate mechanisms governed by Anti-COL2 Antibody and gain valuable insights into its involvement in different biological pathways. The AssayGenie Chicken Anti-COL2 (Collagen Type II) Antibody ELISA Kit provides robust quantitative data for investigating autoimmune pathogenesis, vaccine responses, and disease progression in avian models.
Product Name:
Chicken Anti-COL2 (Collagen Type II) Antibody ELISA Kit
SKU:
AEFI04000
Reactivity:
Chicken
Detection Method:
Indirect
Sensitivity:
0.938 ng/ml
Range:
1.563-100 ng/ml
Sample Type:
Serum, Plasma, Cell Culture Supernatant and Other Biological Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n=5)
82-97%
89-101%
81-96%
EDTA Plasma (n=5)
85-103%
85-93%
95-104%
Heparin Plasma (n=5)
90-102%
92-101%
83-99%
Recovery:
Matrix
Recovery Range (%)
Average (%)
Serum (n=5)
91-104
96
EDTA Plasma (n=5)
89-105
93
Heparin Plasma (n=5)
87-104
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate to room temperature for at least 30 minutes. Designate standard, sample and control wells on the pre-coated plate.
2
Standard, Sample & Control Loading: Aliquot standard solutions, samples and Sample Dilution Buffer into the appropriate wells.
3
First Incubation & Wash: Seal the plate and incubate at 37°C for 30 minutes. Wash 3 times with Wash Buffer.
4
HRP-labelled Antibody & Wash: Add HRP-labelled antibody working solution to each well and incubate at 37°C for 30 minutes. Wash 5 times, allowing buffer to stay 1-2 minutes per wash.
5
Color Development: Add TMB substrate and incubate at 37°C in the dark for 10-20 minutes.
6
Stop Reaction & Reading: Add Stop Solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately.
Plasma
Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed.
Tissue Homogenate
Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant.
Cell Lysate
Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1vial
2vial
Place the test standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
