The Chicken Ghrelin (GHRL) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ghrelin levels in chicken serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for research applications in poultry science and animal nutrition.Ghrelin is a multifunctional hormone that plays a crucial role in regulating food intake, energy balance, and metabolism in chickens. It is also involved in various physiological processes, such as growth hormone secretion, gastric motility, and stress response.
Understanding the levels of ghrelin in chickens can provide valuable insights into their feeding behavior, growth performance, and overall health.With its high-quality components and user-friendly protocol, the Chicken Ghrelin (GHRL) ELISA Kit is an essential tool for studying the role of ghrelin in poultry physiology and developing innovative strategies for enhancing poultry production and welfare.
Product Name:
Chicken Ghrelin (GHRL) ELISA Kit
Product Code:
CHDL00020
Size:
96 Assays
Target:
GHRL
Synonyms:
MTLRP, Growth Hormone-Releasing Peptide
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
