The Chicken Interleukin-13 (IL-13) ELISA Kit is a specialized assay designed for the accurate measurement of IL-13 levels in chicken serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with reliable and reproducible results, making it an invaluable tool for studying the role of IL-13 in various biological processes. Interleukin-13 is a key cytokine involved in regulating immune responses, particularly in promoting allergic inflammation and contributing to the development of asthma and other inflammatory diseases.
By accurately quantifying IL-13 levels, researchers can better understand its role in immune system modulation and potentially identify new therapeutic targets for treating related conditions. The Chicken Interleukin-13 (IL-13) ELISA Kit is a vital resource for researchers looking to delve deeper into the mechanisms underlying immune responses and inflammatory disorders in chickens, ultimately leading to advancements in our understanding of these important biological processes.
Product Name:
Chicken Interleukin 13 (IL-13) ELISA Kit
Product Code:
CHDL00031
Size:
96 Assays
Target:
IL13
Synonyms:
ALRH, BHR1, P600, IL-13, NC30
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
