The Chicken MASP2 (Mannan-Binding Lectin Serine Protease 2) ELISA Kit offered by AssayGenie is a cutting-edge tool designed for the precise measurement of MASP2 levels in chicken samples. This kit boasts exceptional sensitivity and specificity, ensuring the accuracy and reproducibility of results for a variety of research applications. MASP2, a key component of the lectin pathway of the complement system, plays a crucial role in the innate immune response by activating complement cascade upon binding to microbial pathogens.
Understanding the levels of MASP2 in chicken samples is vital for investigating immune responses to infections and diseases, as well as for exploring potential therapeutic interventions. With the Chicken MASP2 ELISA Kit from AssayGenie, researchers can delve deeper into the intricate mechanisms of the chicken immune system and gain valuable insights into host-pathogen interactions. Trust in this advanced ELISA kit to deliver reliable and insightful data for your research needs.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-103%
82-92%
80-96%
EDTA Plasma (n = 5)
87-103%
89-98%
87-99%
Heparin Plasma (n = 5)
92-103%
85-99%
80-92%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-102
93
EDTA Plasma (n = 5)
88-105
96
Heparin Plasma (n = 5)
86-98
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antibody Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
Hernandez et al.
SUPLEMENTACIÓN DE ÁCIDO GUANIDINOACÉTICO EN DIETAS CON NIVELES REDUCIDOS DE ENERGÍA Y SU EFECTO SOBRE EL DESEMPEÑO PRODUCTIVO Y EL RENDIMIENTO EN CANAL DE POLLOS DE ENGORDA
