The Chicken TNF-alpha (Tumor Necrosis Factor-alpha) ELISA Kit from AssayGenie is a specialized assay designed for the precise measurement of TNF-alpha levels in chicken serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for various research purposes. TNF-alpha is a key cytokine involved in the regulation of inflammation and immune response in chickens. Its dysregulation has been implicated in various poultry diseases and conditions, including infections, inflammatory disorders, and autoimmune diseases.
The Chicken TNF-alpha ELISA Kit provides a valuable tool for studying the role of TNF-alpha in chicken health and disease, enabling researchers to explore potential therapeutic interventions and management strategies. With its user-friendly protocol and robust performance, the Chicken TNF-alpha ELISA Kit is an essential tool for researchers and veterinarians seeking to investigate the physiological and pathological functions of TNF-alpha in chickens. Trust AssayGenie's expertise and quality products for accurate and insightful results in your avian research endeavors.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-105%
87-97%
82-99%
EDTA Plasma (n = 5)
85-102%
87-95%
90-99%
Heparin Plasma (n = 5)
91-101%
82-98%
82-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-105
97
EDTA Plasma (n = 5)
85-105
97
Heparin Plasma (n = 5)
93-104
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
