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Key Features

Chk2 Rabbit Monoclonal Antibody (CAB19543)

Datasheet MSDS
SKU CAB19543
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, Chk2
Research Use Cell Death
$173 - $603

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Description

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Chk2 Monoclonal Antibody (CAB19543)

The Chk2 Monoclonal Antibody (CAB19543) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly selective and sensitive for detecting CHK2 in human samples, making it a valuable tool for studies in oncology, genetics, and molecular biology.CHK2 is a tumor suppressor protein that is activated in response to DNA damage, leading to cell cycle arrest and DNA repair.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Chk2 Monoclonal Antibody
SKU:CAB19543
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC57076
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSRE SDVE AQQS HGSS ACSQ PHGS VTQS QGSS SQSQ GISS SSTS TMPN SSQS SHSS SGTL SSLE TVST QELY SIPE DQEP EDQE PEEP TPAP WARL WALQ
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IF/ICC1:200 - 1:800
IHC-P1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, Chk2
Positive Sample:HCT 116, HeLa
Cellular Localization:Nucleus, Pml Body, Nucleoplasm.
Calculated MW:15-38kDa/50-65kDa
Observed MW:61kDa

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID11200
RRIDAB_2862658
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Chk2 Rabbit mAb (CAB19543) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon tissue using Chk2 Rabbit mAb (CAB19543) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Chk2 Rabbit mAb (CAB19543) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageConfocal imaging of HT-29 cells using Chk2 Rabbit mAb (CAB19543, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Product ImageConfocal imaging of HAP1 cells using Chk2 Rabbit mAb (CAB19543, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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