Description
| Product Name: | Cholinesterase (CHE) Activity Assay Kit |
| Product Code: | MAES0210 |
| Sample Type: | Serum, Plasma, Animal Tissue |
| Assay Time: | 60 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | CHE |
| Detection Instrument: | Microplate reader (505-535 nm, optimum wavelength: 520 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 2.17 U/mL |
| Detection Range: | 2.17-71.33 U/mL |
| Inter-assay CV: | 0.064 |
| Intra-assay CV: | 0.024 |
Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
| Storage: | 2-8°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | EP tube, centrifuge, Vortex mixer, 37℃ water bath, Microplate reader |
