The CISH Polyclonal Antibody (CAB6286) is a valuable tool for research into the CISH protein, a crucial negative regulator of cytokine signaling. As an essential component of the suppressor of cytokine signaling (SOCS) family, CISH plays a key role in modulating immune responses and maintaining immune homeostasis. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blotting. By binding to the CISH protein, researchers can accurately detect and analyze its expression in different cell types.
This makes the CISH Polyclonal Antibody ideal for studies in immunology, inflammation, and infectious diseases.The importance of CISH in regulating cytokine signaling pathways makes it a relevant target for research into autoimmune disorders, chronic inflammatory conditions, and infectious diseases. By understanding the function of CISH, researchers can gain insights that may lead to the development of novel therapeutic strategies to modulate immune responses and treat associated diseases effectively.
Product Name:
CISH Rabbit Polyclonal Antibody
SKU:
CAB6286
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human CISH (NP_659508.1).
The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1154
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CISH antibody (CAB6286) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
