Western blot analysis of GSDMB-C-6-his protein, using Cleaved Gasdermin B（C Terminal） Rabbit mAb (CAB24342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features
Cleaved Gasdermin B (C Terminal) Monoclonal Antibody (CAB24342)
Cleaved Gasdermin B (C Terminal) Monoclonal Antibody (CAB24342)
The Cleaved Gasdermin B (C Terminal) Monoclonal Antibody (CAB24342) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, is highly specific and reactive with cleaved Gasdermin B in human samples, providing researchers with a reliable tool for Western blot applications.Gasdermin B is a critical mediator of pyroptosis, a process that leads to the release of pro-inflammatory cytokines and cell death. Understanding the cleavage and activation of Gasdermin B is essential for studying inflammatory pathways and developing targeted therapies for conditions such as infectious diseases and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cleaved Gasdermin B (C Terminal) Monoclonal Antibody
SKU:
CAB24342
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC63400
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GKSL G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
GSDMB-C-6-his, 293T GSDMB stable cells transfected with GZMA
Cellular Localization:
Cytoplasm.
Calculated MW:
18kDa/45kDa/46kDa/47kDa
Observed MW:
18kDa
This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.
Purification Method
Affinity purification
Gene ID
55876
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B（C Terminal） Rabbit mAb (CAB24342) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.3s. WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B（C Terminal） Rabbit mAb (CAB24342) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
![Anti-Cleaved C-terminal GSDMD [R08-5D3] Monoclonal Antibody (AGMB03938)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275227/677576/anti-cleaved-c-terminal-gsdmd-r08-5d3-monoclonal-antibody-agmb03938__33976.1773033034.jpg?c=2 "Anti-Cleaved C-terminal GSDMD [R08-5D3] Monoclonal Antibody (AGMB03938)")
![Anti-Cleaved C-terminal GSDMD [R09-7E6] Monoclonal Antibody (AGMB03939)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275228/676098/anti-cleaved-c-terminal-gsdmd-r09-7e6-monoclonal-antibody-agmb03939__35476.1773028350.jpg?c=2 "Anti-Cleaved C-terminal GSDMD [R09-7E6] Monoclonal Antibody (AGMB03939)")
![Anti-Cleaved N-terminal GSDMD [R07-2C4] Monoclonal Antibody (AGMB03937)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275226/676358/anti-cleaved-n-terminal-gsdmd-r07-2c4-monoclonal-antibody-agmb03937__72904.1773029184.jpg?c=2 "Anti-Cleaved N-terminal GSDMD [R07-2C4] Monoclonal Antibody (AGMB03937)")