| Kit Name: | CLO (Clorprenaline) ELISA Kit |
| Kit Size: | 96 Assays |
| Test Principle: | This kit uses Competitive-ELISA as the method. It can be used to detect Clorprenaline (CLO) in samples, such as serum, urine, tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CLO in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CLO antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of CLO. The concentration of CLO in the samples can be calculated by comparing the OD of the samples to the standard curve. |
| Sensitivity: | 0.05 ppb (ng/mL) |
| Reaction Mode: | 25°C,30 min~15 min |
| Detection Limit: | Serum --- 0.25 ppb; urine, Tissue --- 0.5 ppb |
| Cross Reactivity: | | Clorprenaline | 100% | | Tulobuterol | 91% | | Dobutamine, Mabuterol, Ractopamine | <0.1% | | Clenproperol, Cimaterol, Cimbuterol | <0.1% | | Clenpenterol, Mapenterol, Terbutaline | <0.1% | | Bromchlorbuterol, Salbutamol, Clenbuterol | <0.1% | | Brombuterol, Carbuterol | <0.1% | |
| Sample Recovery Rate: | 100%±30%. |
| Kits Components: | | Item | Specifications | | ELISA Microtiter plate | 96 wells | | Standard Liquid | 1.5 mL each (0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05ppb) | | Sample Dilution | 30 mL | | 11×Concentrated HRP Conjugate Solution | 1 mL | | HRP Conjugate Dilution | 10 mL | | Substrate Reagent A | 7 mL | | Substrate Reagent B | 7 mL | | Stop Solution | 7 mL | | 20×Concentrated Wash Buffer | 25 mL | | Plate Sealer | 3 pieces | | Sealed Bag | 1 piece | | Manual | 1 copy | |
| Instruments: | Microplate Reader, Homogenizer, water bath, Oscillators, Centrifuge, Balance (sensibility 0.01 g). |
| Micropipette: | Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL). |
| Reagents: | Contact Technical Support |
| Storage And Expiry Date: | Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box. |
