Description
Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His)
Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly1834-Ile2099, with an N-terminal His tag. Predicted molecular weight: 27.36 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. For research use only.
TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.
| Product Name: | Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His) |
| SKU: | GXRP03326 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Clostridioides difficile |
| Synonyms: | Toxin B, Glucosyltransferase TcdB, tcdB, toxB, Clostridium difficile Toxin |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly1834-Ile2099 |
| Calculated MW: | 27.36 kDa |
| UniProt: | P18177 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
