Anti-COG6 Antibody (CAB10319)

SKU:
CAB10319
€99 - €249

Description

Technical Manual

Anti-COG6 Antibody

Product Name:Anti-COG6 Antibody
Product SKU:CAB10319
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi apparatus. The encoded protein is organized with conserved oligomeric Golgi complex components 5, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 508-657 of human COG6 (NP_065802.1).
Sequence:

MKTTLALFEFTDRRLEMLQFQIEAHLDTLINEQASYVLTRVGLSYIYNTVQQHKPEQGSLANMPNLDSVTLKAAMVQFDRYLSAPDNLLIPQLNFLLSATVKEQIVKQSTELVCRAYGEVYAAVMNPINEYKDPENILHRSPQQVQTLLS

Gene ID:57511
Swiss Prot:Q9Y2V7
Synonyms:COG6; CDG2L; COD2; SHNS
Calculated MW:7kDa/68kDa/73kDa
Observed MW:73kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Jurkat, A-549, U-87MG, Mouse kidney, Rat testis
Cellular Location:Golgi apparatus membrane, Peripheral membrane protein

Product Images

Anti-COG6 Antibody (CAB10319)

Western blot analysis of extracts of various cell lines, using COG6 antibody (CAB10319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Uniprot Information

UniProt Protein Function:COG6: Required for normal Golgi function. Defects in COG6 are the cause of congenital disorder of glycosylation type 2L (CDG2L). CDG2L is a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Clinical features of CDG2L include neonatal intractable focal seizures, vomiting, loss of consciousness, intracranial bleeding due to vitamin K deficiency, and death in infancy. Belongs to the COG6 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: Golgi membrane; Golgi transport complex; trans-Golgi network membrane

Molecular Function:protein binding

Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport

Disease: Congenital Disorder Of Glycosylation, Type Iil; Shaheen Syndrome

NCBI Summary:This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi apparatus. The encoded protein is organized with conserved oligomeric Golgi complex components 5, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
UniProt Code:Q9Y2V7
NCBI GenInfo Identifier:182676410
NCBI Gene ID:57511
NCBI Accession:Q9Y2V7.2
UniProt Secondary Accession:Q9Y2V7,Q5T0U1, Q6AI19, Q86V49, Q9ULT5,
UniProt Related Accession:Q9Y2V7
Molecular Weight:73kDa
NCBI Full Name:Conserved oligomeric Golgi complex subunit 6
NCBI Synonym Full Names:component of oligomeric golgi complex 6
NCBI Official Symbol:COG6
NCBI Official Synonym Symbols:COD2; SHNS; CDG2L
NCBI Protein Information:conserved oligomeric Golgi complex subunit 6
UniProt Protein Name:Conserved oligomeric Golgi complex subunit 6
UniProt Synonym Protein Names:Component of oligomeric Golgi complex 6
Protein Family:Conserved oligomeric Golgi complex
UniProt Gene Name:COG6
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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