The Collagen X/COL10A1 Monoclonal Antibody (CAB11645) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced rabbit monoclonal technology, exhibits high specificity and sensitivity towards human collagen X samples, making it an ideal choice for experiments such as Western blotting.Collagen X plays a vital role in bone formation and growth plate development, making it a key protein of interest in orthopedic and musculoskeletal research. By targeting collagen X with this monoclonal antibody, researchers can accurately detect and analyze its expression levels in a variety of cell types, providing valuable insights into skeletal development and potential disease mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Collagen X/COL10A1 Monoclonal Antibody
SKU:
CAB11645
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0659
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
Purification Method
Affinity purification
Gene ID
1300
RRID
AB_2861617
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Collagen X/COL10A1 Rabbit mAb (CAB11645) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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