The CORT (Corticosterone) ELISA Kit offered by Assay Genie is a powerful tool for accurately measuring corticosterone levels in various biological samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it an invaluable resource for a wide range of research applications. Corticosterone is a key hormone involved in the stress response and plays a critical role in regulating various physiological processes in the body. Dysregulation of corticosterone levels has been linked to numerous health conditions such as stress-related disorders, metabolic disorders, and immune dysfunction, highlighting the importance of monitoring and studying this hormone for potential therapeutic interventions.
By using the CORT ELISA Kit from Assay Genie, researchers can gain valuable insights into corticosterone levels in different biological samples, paving the way for a better understanding of its role in health and disease. Get your kit today and advance your research efforts in the field of endocrinology and stress-related disorders.
Product Name:
CORT (Corticosterone) ELISA Kit
SKU:
UNFI0096
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
1.688 ng/mL
Range:
2.813-180 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
85-96%
87-103%
82-95%
EDTA Plasma (n = 10)
80-95%
80-100%
80-93%
Heparin Plasma (n = 10)
89-105%
88-103%
83-92%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
88-104
94
EDTA Plasma (n = 10)
85-105
94
Heparin Plasma (n = 10)
95-105
100
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
