Anti-COX5A Antibody (CAB6437)

Antibody Name:	Anti-COX5A Antibody
Antibody SKU:	CAB6437
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, mouse skeletal muscle, mouse testis, mouse heart, mouse brain, rat heart, rat kidney, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLGA ALRR CAVA ATTR ADPR GLLH SART PGPA VAIQ SVRC YSHG SQET DEEF DARW VTYF NKPD IDAW ELRK GINT LVTY DMVP EPKI IDAA LRAC RRLN DFAS TVRI LEVV KDKA GPHK EIYP YVIQ ELRP TLNE LGIS TPEE LGLD KV
Gene ID:	9377
Uniprot:	P20674
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	16kDa
Observed MW:	14kDa

Synonyms:

COX5A, COX, COX-VA, VA

Background:

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

UniProt Protein Function:	COX5A: This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5A family.
UniProt Protein Details:	Protein type:EC 1.9.3.1; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: mitochondrial inner membrane Molecular Function:cytochrome-c oxidase activity; electron carrier activity; metal ion binding Biological Process: cellular metabolic process; transmembrane transport
NCBI Summary:	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]
UniProt Code:	P20674
NCBI GenInfo Identifier:	218511986
NCBI Gene ID:	9377
NCBI Accession:	P20674.2
UniProt Secondary Accession:	P20674,P30045, Q8TB65,
UniProt Related Accession:	P20674
Molecular Weight:	150
NCBI Full Name:	Cytochrome c oxidase subunit 5A, mitochondrial
NCBI Synonym Full Names:	cytochrome c oxidase subunit Va
NCBI Official Symbol:	COX5A
NCBI Official Synonym Symbols:	VA; COX; COX-VA
NCBI Protein Information:	cytochrome c oxidase subunit 5A, mitochondrial; cytochrome c oxidase polypeptide Va; mitochondrial cytochrome c oxidase subunit Va; cytochrome c oxidase polypeptide, mitochondrial
UniProt Protein Name:	Cytochrome c oxidase subunit 5A, mitochondrial
UniProt Synonym Protein Names:	Cytochrome c oxidase polypeptide Va
Protein Family:	Cytochrome c oxidase
UniProt Gene Name:	COX5A
UniProt Entry Name:	COX5A_HUMAN

	Western blot analysis of extracts of various cell lines, using COX5A Antibody (CAB6437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat ovary using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using COX5A antibody (CAB6437). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
COX5A Antibody (PACO05752)	Human COX5A ELISA Kit
COX5A Antibody (PACO08547)	Human Cytochrome c oxidase subunit 5A, mitochondrial (COX5A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)