The COX6B1 Antibody (CAB12425) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting. By binding to the COX6B1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in mitochondrial biology and related fields.COX6B1 is a crucial subunit of cytochrome c oxidase, which plays a pivotal role in the production of ATP, the energy currency of cells. Dysfunction of this enzyme complex has been linked to various human diseases, including mitochondrial disorders and neurodegenerative conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
COX6B1 Antibody
SKU:
CAB12425
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COXG, COX6B, MC4DN7, COXVIb1, COX6B1
Positive Sample:
Mouse brain, Mouse liver, Rat liver
Cellular Localization:
Mitochondrion Intermembrane Space.
Calculated MW:
10kDa
Observed MW:
12kDa
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively.
Purification Method
Affinity purification
Gene ID
1340
RRID
AB_2759267
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COX6B1 Rabbit pAb (CAB12425) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
