Anti-CRABP2 Antibody (CAB6119)

Antibody Name:	Anti-CRABP2 Antibody
Antibody SKU:	CAB6119
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-94 of human CRABP2 (NP_001869.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, A-431, 22Rv1, HT-29, 293T, Mouse brain, Mouse uterus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-94 of human CRABP2 (NP_001869.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VLGV NVML RKIA VAAA SKPA VEIK QEGD TFYI KTST TVRT TEIN FKVG EEFE EQTV DGRP CKSL VKWE SENK M
Gene ID:	1382
Uniprot:	P29373
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Nucleus
Calculated MW:	15kDa
Observed MW:	15kDa

Synonyms:

CRABP2, CRABP-II, RBP6

Background:

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	CRABP2: Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; cytosol Molecular Function:retinoid binding; retinoic acid binding; transporter activity; retinal binding; retinol binding Biological Process: embryonic forelimb morphogenesis; epidermis development; regulation of transcription, DNA-dependent; retinoic acid metabolic process; signal transduction; transmembrane transport
NCBI Summary:	This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010]
UniProt Code:	P29373
NCBI GenInfo Identifier:	132401
NCBI Gene ID:	1382
NCBI Accession:	P29373.2
UniProt Secondary Accession:	P29373,Q6ICN6, B2R4Z8, D3DVC5, F1T098,
UniProt Related Accession:	P29373
Molecular Weight:	138
NCBI Full Name:	Cellular retinoic acid-binding protein 2
NCBI Synonym Full Names:	cellular retinoic acid binding protein 2
NCBI Official Symbol:	CRABP2
NCBI Official Synonym Symbols:	RBP6; CRABP-II
NCBI Protein Information:	cellular retinoic acid-binding protein 2; cellular retinoic acid-binding protein II
UniProt Protein Name:	Cellular retinoic acid-binding protein 2
UniProt Synonym Protein Names:	Cellular retinoic acid-binding protein II; CRABP-II
Protein Family:	Cellular retinoic acid-binding protein
UniProt Gene Name:	CRABP2
UniProt Entry Name:	RABP2_HUMAN

	Western blot analysis of extracts of various cell lines, using CRABP2 antibody (CAB6119) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using CRABP2 antibody (CAB6119) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CRABP2 antibody (CAB6119). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CRABP2 Antibody (PACO00635)	Human CRABP2 ELISA Kit
CRABP2 Antibody (PACO08585)	Human Cellular retinoic acid-binding protein 2 (CRABP2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)