The Casein Kinase 2 alpha (CSNK2A1) Antibody (CAB1014) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for CSNK2A1 in human samples, making it an ideal choice for Western blot applications.CSNK2A1, also known as CK2α, is a protein kinase involved in diverse cellular processes including cell growth, proliferation, and survival. Dysregulation of CSNK2A1 has been linked to various diseases such as cancer, neurological disorders, and metabolic diseases, highlighting its importance as a therapeutic target.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Casein Kinase 2 alpha (CSNK2A1) Antibody
SKU:
CAB1014
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
SGC-7901, H460, HT-1080, BT-474, SW620, Mouse liver, Mouse heart, Rat liver, Mouse heart, Rat liver, SGC-7901
Cellular Localization:
Nucleus.
Calculated MW:
45kDa
Observed MW:
41kDa
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1457
RRID
AB_2757666
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (CAB1014) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (CAB1014) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (CAB1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (CAB1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
