Anti-CTCF Antibody (CAB1133)

Antibody Name:	Anti-CTCF Antibody
Antibody SKU:	CAB1133
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 ChIP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	293T, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEGD AVEA IVEE SETF IKGK ERKT YQRR REGG QEED ACHL PQNQ TDGG EVVQ DVNS SVQM VMME QLDP TLLQ MKTE VMEG TVAP EAEA AVDD TQII TLQV VNME EQPI NIGE LQLV QVPV PVTV PVAT TSVE ELQG AYEN EVSK EGLA ESEP MICH TLPL PEGF QVVK VGAN GEVE TLEQ GELP PQED PSWQ KDPD YQPP AKKT KKTK KSKL RYTE EGKD VDVS VYDF EEEQ QEGL LSEV NAEK VVGN MKPP KPTK IKKK
Gene ID:	10664
Uniprot:	P49711
Cellular Location:	Chromosome, Nucleus, centromere, nucleoplasm
Calculated MW:	45kDa/82kDa
Observed MW:	140kDa

Synonyms:

CTCF, MRD21, CTCF

Background:

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.
UniProt Protein Details:	Protein type:Tumor suppressor; C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 16q21-q22.3 Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding; chromatin insulator sequence binding; transcription factor activity; transcription corepressor activity Biological Process: genetic imprinting; transcription, DNA-dependent; maintenance of DNA methylation; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent Disease: Mental Retardation, Autosomal Dominant 21
NCBI Summary:	This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	P49711
NCBI GenInfo Identifier:	1706179
NCBI Gene ID:	10664
NCBI Accession:	P49711.1
UniProt Related Accession:	P49711
Molecular Weight:	83; 84; 84kDa
NCBI Full Name:	Transcriptional repressor CTCF
NCBI Synonym Full Names:	CCCTC-binding factor
NCBI Official Symbol:	CTCFÂ Â
NCBI Official Synonym Symbols:	MRD21Â Â
NCBI Protein Information:	transcriptional repressor CTCF
UniProt Protein Name:	Transcriptional repressor CTCF
UniProt Synonym Protein Names:	11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
Protein Family:	Transcriptional repressor
UniProt Gene Name:	CTCFÂ Â
UniProt Entry Name:	CTCF_HUMAN

	Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Western blot analysis of extracts of NIH/3T3 cells, using CTCF antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Mouse brain using CTCF Rabbit pAb at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using CTCF Rabbit pAb at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon using CTCF Rabbit pAb at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using CTCF Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using CTCF Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using CTCF Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CTCF antibody at a dilition of 1:500.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies	ELISA Kits
Anti-CTCF Antibody (CAB13272)	Human Transcriptional repressor CTCF (CTCF) ELISA Kit
Anti-CTCF Antibody (CAB18627)[KO Validated]
Anti-CTCF Antibody [KO Validated] (CAB19588)
CTCF Antibody (PACO08653)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)