Anti-CTCFL Antibody (CAB6149)

Antibody Name:	Anti-CTCFL Antibody
Antibody SKU:	CAB6149
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HeLa, Mouse heart, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAT EISV LSEQ FTKI KELE LMPE KGLK EEEK DGVC REKD HRSP SELE AERT SGAF QDSV LEEE VELV LAPS EESE KYIL TLQT VHFT SEAV ELQD MSLL SIQQ QEGV QVVV QQPG PGLL WLEE GPRQ SLQQ CVAI SIQQ ELYS PQEM EVLQ FHAL EENV MVAS EDSK LAVS LAET TGLI KLEE EQEK NQLL AERT KEQL FFVE TMSG DERS DEIV LTVS NSNV EEQE DQPT AGQA DAEK AKST KNQR KTKG AKGT FHCD VCMF TSSR MS
Gene ID:	140690
Uniprot:	Q8NI51
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	35kDa/46-80kDa
Observed MW:	68kDa

Synonyms:

CTCFL, BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2

Background:

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	BORIS: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3. Testis specific. Specifically expressed in primary spermatocytes. Belongs to the CTCF zinc-finger protein family.
UniProt Protein Details:	Protein type:DNA-binding; Cancer Testis Antigen (CTA); C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; sequence-specific DNA binding; metal ion binding Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; DNA methylation during gametogenesis; positive regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; cell cycle
UniProt Code:	Q8NI51
NCBI GenInfo Identifier:	313104098
NCBI Gene ID:
NCBI Accession:	Q8NI51.2
Molecular Weight:
NCBI Full Name:	Transcriptional repressor CTCFL
UniProt Protein Name:	Transcriptional repressor CTCFL
UniProt Synonym Protein Names:	Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein; Cancer/testis antigen 27; CT27; Zinc finger protein CTCF-T
Protein Family:	Transcriptional repressor
UniProt Gene Name:	CTCFL
UniProt Entry Name:	CTCFL_HUMAN

	Western blot analysis of extracts of various cell lines, using CTCFL antibody (CAB6149) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using CTCFL antibody (CAB6149). Blue: DAPI for nuclear staining.

ELISA Kits

Human CTCFL ELISA Kit

Human Transcriptional repressor CTCFL (CTCFL) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)