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Cell Biology Antibodies 17 Anti-Cullin4A Antibody CAB5064
Key Features

Cullin 4A Rabbit Monoclonal Antibody (CAB5064)

Datasheet MSDS
SKU CAB5064
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CUL4A, cullin-4A, Cullin 4A
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Cullin 4A Monoclonal Antibody (CAB5064)

The Cullin 4A Monoclonal Antibody (CAB5064) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in rabbits, offers high specificity and sensitivity for detecting Cullin4A in various cell types and tissues.Cullin4A is a critical component of the Cullin-RING E3 ubiquitin ligase complex, which plays a crucial role in protein degradation and cell cycle control. Dysregulation of Cullin4A has been linked to various diseases, including cancer, making it a potential target for therapeutic interventions.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Cullin 4A Monoclonal Antibody
SKU:CAB5064
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC1237
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:FKHK LFRI KINQ IQMK ETVE EQVS TTER VFQD RQYQ IDAA IVRI MKMR KTLG HNLL VSEL YNQL KFPV KPGD LKKR IESL IDRD YMER DKDN PNQY HYVA
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CUL4A, cullin-4A, Cullin 4A
Positive Sample:HeLa, Raji
Cellular Localization:Nucleoplasm, Nucleus.
Calculated MW:88kDa
Observed MW:88kDa

CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).

Purification MethodAffinity purification
Gene ID8451
RRIDAB_2863427
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Cullin 4A Rabbit mAb (CAB5064) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using Cullin 4A Rabbit mAb (CAB5064) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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