Anti-CXCR3 Antibody (CAB2939)

Antibody Name:	Anti-CXCR3 Antibody
Antibody SKU:	CAB2939
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-368 of human CXCR3 (NP_001495.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	293T, K-562, HepG2, SGC996, SW620

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-368 of human CXCR3 (NP_001495.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2833
Uniprot:	P49682
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	28kDa/40kDa/45kDa
Observed MW:	44kDa

Synonyms:

CXCR3, CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR

Background:

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716).

UniProt Protein Function:	CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: Xq13 Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane Molecular Function:C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity Biological Process: positive regulation of chemotaxis; apoptosis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; negative regulation of angiogenesis; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; negative regulation of endothelial cell proliferation; positive regulation of cell proliferation; regulation of leukocyte migration; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; cell motility; cell adhesion
NCBI Summary:	This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]
UniProt Code:	P49682
NCBI GenInfo Identifier:	2829400
NCBI Gene ID:	2833
NCBI Accession:	P49682.2
UniProt Secondary Accession:	P49682,O15185, Q7Z710, Q9P2T4, Q9P2T5, B2R982,
UniProt Related Accession:	P49682
Molecular Weight:	Observed MW: 44kDaCalculated MW: 28kDa/40kDa/45kDa
NCBI Full Name:	C-X-C chemokine receptor type 3
NCBI Synonym Full Names:	chemokine (C-X-C motif) receptor 3
NCBI Official Symbol:	CXCR3
NCBI Official Synonym Symbols:	GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
NCBI Protein Information:	C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
UniProt Protein Name:	C-X-C chemokine receptor type 3
UniProt Synonym Protein Names:	CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD_antigen: CD183
Protein Family:	C-X-C chemokine receptor
UniProt Gene Name:	CXCR3
UniProt Entry Name:	CXCR3_HUMAN

	Western blot analysis of extracts of various cell lines, using CXCR3 antibody (CAB2939) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using CXCR3 antibody (CAB2939) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using CXCR3 Antibody (CAB2939) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse skeletal muscle using CXCR3 Antibody (CAB2939) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CXCR3 Antibody (CAB2939) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Human CD183/CXCR3 Monoclonal Antibody (FITC Conjugated) [G025H7](AGEL1794)	Human CXCR3 / CD183 ELISA Kit
Human CD183/CXCR3 Monoclonal Antibody (PE Conjugated) [G025H7](AGEL1795)	Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit (HUES00515)
Human CD183/CXCR3 Monoclonal Antibody (APC Conjugated) [G025H7](AGEL1796)	Human CXCR3 (Chemokine CXC Receptor 3) ELISA Kit (HUES01985)
Anti-CXCR3 Antibody (CAB11601)	Human C-X-C chemokine receptor type 3 (CXCR3) ELISA Kit
Anti-CXCR3 Antibody (CAB11294)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)