Anti-CXXC1 Antibody (CAB13423)

Antibody Name:	Anti-CXXC1 Antibody
Antibody SKU:	CAB13423
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human CXXC1 (NP_001095124.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	HepG2, BT-474, HepG2, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human CXXC1 (NP_001095124.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEGD GSDP EPPD AGED SKSE NGEN APIY CICR KPDI NCFM IGCD NCNE WFHG DCIR ITEK MAKA IREW YCRE CREK DPKL EIRY RHKK SRER DGNE RDSS EPRD EGGG RKRP VPDP DLQR RAGS GTGV GAML ARGS ASPH KSSP QPLV ATPS QHHQ QQQQ QIKR SARM CGEC EACR RTED CGHC DFCR DMKK FGGP NKIR QKCR LRQC QL
Gene ID:	30827
Uniprot:	Q9P0U4
Cellular Location:	Nucleus speckle
Calculated MW:	75kDa/76kDa
Observed MW:	80kDa

Synonyms:	CXXC1, 2410002I16Rik, 5830420C16Rik, CFP1, CGBP, HsT2645, PCCX1, PHF18, SPP1, ZCGPC1, hCGBP
Background:	This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development.

UniProt Protein Function:	CXXC1: Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 18q12 Cellular Component: cytoplasm; histone methyltransferase complex; nuclear speck; nucleoplasm; nucleus Molecular Function:histone lysine N-methyltransferase activity (H3-K4 specific); protein binding; unmethylated CpG binding Biological Process: histone H3-K4 methylation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development. [provided by RefSeq, Sep 2015]
UniProt Code:	Q9P0U4
NCBI GenInfo Identifier:	20138037
NCBI Gene ID:	30827
NCBI Accession:	Q9P0U4.2
UniProt Secondary Accession:	Q9P0U4,Q8N2W4, Q96BC8, Q9P2V7, B2RC03,
UniProt Related Accession:	Q9P0U4
Molecular Weight:	76,227 Da
NCBI Full Name:	CXXC-type zinc finger protein 1
NCBI Synonym Full Names:	CXXC finger protein 1
NCBI Official Symbol:	CXXC1
NCBI Official Synonym Symbols:	CFP1; CGBP; SPP1; PCCX1; PHF18; hCGBP; ZCGPC1; HsT2645; 2410002I16Rik; 5830420C16Rik
NCBI Protein Information:	CXXC-type zinc finger protein 1
UniProt Protein Name:	CXXC-type zinc finger protein 1
UniProt Synonym Protein Names:	CpG-binding protein; PHD finger and CXXC domain-containing protein 1
Protein Family:	CXXC-type zinc finger protein
UniProt Gene Name:	CXXC1
UniProt Entry Name:	CXXC1_HUMAN

	Western blot analysis of extracts of various cell lines, using CXXC1 antibody (CAB13423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of A-549 cells using CXXC1 antibody (CAB13423).
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 3ug CXXC1 antibody (CAB13423). Western blot was performed from the immunoprecipitate using CXXC1 antibody (CAB13423) at a dilition of 1:500.

Antibodies

Anti-CXXC1 Antibody (CAB13424)

Anti-CXXC1 Antibody (CAB5814)

CXXC1 Antibody (PACO08694)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)