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Anti-CYP11A1 Antibody (CAB1713)

SKU:
CAB1713
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-CYP11A1 Antibody CAB1713
  • Metabolism Antibodies 2 Anti-CYP11A1 Antibody CAB1713
€139 - €419
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Description

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Antibody Name:Anti-CYP11A1 Antibody
Antibody SKU:CAB1713
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CYP11A1 (NP_000772.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:20 - 1:50 IF 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:mouse testis, rat ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CYP11A1 (NP_000772.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ISTR SPRP FNEI PSPG DNGW LNLY HFWR ETGT HKVH LHHV QNFQ KYGP IYRE KLGN VESV YVID PEDV ALLF KSEG PNPE RFLI PPWV AYHQ YYQR PIGV LLKK SAAW KKDR VALN QEVM APEA TKNF LPLL DAVS RDFV SVLH RRIK KAGS GNYS GDIS DDLF RFAF ESIT NVIF GERQ GMLE EVVN PEAQ RFID AIYQ MFHT SVPM LNLP PDLF RLFR TKTW KDHV AAWD VIFS KADI YTQN FYWE LRQK GSVH HDYR GILY RLLG DSKM SFED IKAN V
Gene ID:1583
Uniprot:P05108
Cellular Location:Mitochondrion membrane
Calculated MW:42kDa/60kDa
Observed MW:50kDa
Synonyms:CYP11A1, CYP11A, CYPXIA1, P450SCC
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.
UniProt Protein Function:CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Mitochondrial; Lipid Metabolism - C21-steroid hormone; Oxidoreductase; EC 1.14.15.6

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; perikaryon

Molecular Function:cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; cholesterol binding; heme binding

Biological Process: response to alkaloid; steroid metabolic process; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; estrogen biosynthetic process; response to L-ascorbic acid; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; response to gamma radiation; cerebellum development; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; fractalkine metabolic process; phthalate metabolic process; hippocampus development; mating behavior; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process

Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
UniProt Code:P05108
NCBI GenInfo Identifier:143811381
NCBI Gene ID:1583
NCBI Accession:P05108.2
UniProt Secondary Accession:P05108,Q15081, Q16805, Q8N1A7, A8K8D5, B3KPU8, G3XAD7
UniProt Related Accession:P05108
Molecular Weight:521
NCBI Full Name:Cholesterol side-chain cleavage enzyme, mitochondrial
NCBI Synonym Full Names:cytochrome P450, family 11, subfamily A, polypeptide 1
NCBI Official Symbol:CYP11A1  
NCBI Official Synonym Symbols:CYP11A; CYPXIA1; P450SCC  
NCBI Protein Information:cholesterol side-chain cleavage enzyme, mitochondrial; steroid 20-22-lyase; cytochrome P450 11A1; cytochrome P450(scc); cytochrome P450C11A1; cholesterol 20-22 desmolase; cholesterol monooxygenase (side-chain cleaving); cytochrome P450, subfamily XIA (cholesterol side chain cleavage)
UniProt Protein Name:Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Synonym Protein Names:CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
Protein Family:Cholesterol side-chain cleavage enzyme
UniProt Gene Name:CYP11A1  
UniProt Entry Name:CP11A_HUMAN
Anti-CYP11A1 Antibody (CAB1713)Western blot analysis of extracts of various cell lines, using CYP11A1 antibody (CAB1713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-CYP11A1 Antibody (CAB1713)Immunohistochemistry of paraffin-embedded Human colon using CYP11A1 Rabbit pAb (CAB1713) at dilution of 1:100 (40x lens).

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