Anti-CYP2B6 Antibody (CAB1463)

Antibody Name:	Anti-CYP2B6 Antibody
Antibody SKU:	CAB1463
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, HT-29, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SKGA LMDP TFLF QSIT ANII CSIV FGKR FHYQ DQEF LKML NLFY QTFS LISS VFGQ LFEL FSGF LKYF PGAH RQVY KNLQ EINA YIGH SVEK HRET LDPS APKD LIDT YLLH MEKE KSNA HSEF SHQN LNLN TLSL FFAG TETT STTL RYGF LLML KYPH VAER VYRE IEQV IGPH RPPE L
Gene ID:	1555
Uniprot:	P20813
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:	33kDa/56kDa
Observed MW:	66kDa

Synonyms:

CYP2B6, CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, EFVM, IIB1, P450

Background:

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

UniProt Protein Function:	CYP2B6: cytochrome P450, subfamily IIB. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Phosphorylation is accompanied by a decrease in enzyme activity.
UniProt Protein Details:	Protein type:Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Lipid Metabolism - arachidonic acid Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: endoplasmic reticulum membrane Molecular Function:arachidonic acid epoxygenase activity; heme binding; monooxygenase activity; oxygen binding; steroid hydroxylase activity Biological Process: drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; ketone metabolic process; steroid metabolic process; xenobiotic metabolic process Disease: Efavirenz, Poor Metabolism Of
NCBI Summary:	This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
UniProt Code:	P20813
NCBI GenInfo Identifier:	117205
NCBI Gene ID:	1555
NCBI Accession:	P20813.1
UniProt Secondary Accession:	P20813,Q2V565, Q9UK46, B4DWP3,
UniProt Related Accession:	P20813
Molecular Weight:	33,385 Da
NCBI Full Name:	Cytochrome P450 2B6
NCBI Synonym Full Names:	cytochrome P450 family 2 subfamily B member 6
NCBI Official Symbol:	CYP2B6
NCBI Official Synonym Symbols:	CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
NCBI Protein Information:	cytochrome P450 2B6
UniProt Protein Name:	Cytochrome P450 2B6
UniProt Synonym Protein Names:	1,4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1
Protein Family:	Cytochrome
UniProt Gene Name:	CYP2B6
UniProt Entry Name:	CP2B6_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP2B6 antibody (CAB1463) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
CYP2B6 Antibody (PACO00664)	Human CYP2B6 / Cytochrome P450 2B6 protein ELISA Kit
CYP2B6 Antibody (PACO02541)	Human Cytochrome P450 2B6 (CYP2B6) ELISA Kit
	Cytochrome P450 2B6 Colorimetric Cell-Based ELISA

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)