The Cytokeratin 20 (KRT20) Monoclonal Antibody (CAB19041) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is recommended for use in immunohistochemistry and Western blot applications, providing accurate detection and analysis of Cytokeratin 20 protein expression in various tissue samples.Cytokeratin 20 is commonly used as a marker for detecting gastrointestinal adenocarcinomas and other cancers originating from epithelial cells. Its differential expression in various cancer types makes it a valuable tool for cancer research and diagnosis.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Cytokeratin 20 (KRT20) Monoclonal Antibody
SKU:
CAB19041
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC0288
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
Purification Method
Affinity purification
Gene ID
54474
RRID
AB_2862534
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HT-29 cells, using Cytokeratin 20 (CK20) Rabbit mAb (CAB19041) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat liver, using Cytokeratin 20 (CK20) Rabbit mAb (CAB19041) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Cytokeratin 20 (CK20) Rabbit mAb (CAB19041) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Cytokeratin 20 (CK20) Rabbit mAb (CAB19041) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
