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Anti-DDAH2 Antibody (CAB6457)

SKU:
CAB6457
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 10 Anti-DDAH2 Antibody CAB6457
  • Cell Biology Antibodies 10 Anti-DDAH2 Antibody CAB6457
€139 - €419
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Description

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Antibody Name:Anti-DDAH2 Antibody
Antibody SKU:CAB6457
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, BT-474, MCF7, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGTP GEGL GRCS HALI RGVP ESLA SGEG AGAG LPAL DLAK AQRE HGVL GGKL RQRL GLQL LELP PEES LPLG PLLG DTAV IQGD TALI TRPW SPAR RPEV DGVR KALQ DLGL RIVE IGDE NATL DGTD VLFT GREF FVGL SKWT NHRG AEIV ADTF RDFA VSTV PVSG PSHL RGLC GMGG PRTV VAGS SDAA QKAV RAMA VLTD HPYA SLTL PDDA AADC LFLR PGLP GVPP FLLH RGGG DLPN SQEA LQKL SDVT LVPV SCSE LEKA GAGL SSLC LVLS TRPH S
Gene ID:23564
Uniprot:O95865
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:29kDa
Observed MW:30kDa
Synonyms:DDAH2, DDAH, DDAHII, G6a, HEL-S-277, NG30
Background:This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
UniProt Protein Function:DDAH2: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family.
UniProt Protein Details:

Protein type:EC 3.5.3.18; Apoptosis; Hydrolase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: centrosome; mitochondrion; cytoplasm

Molecular Function:amino acid binding; protein binding; dimethylargininase activity; catalytic activity

Biological Process: positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; arginine catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; negative regulation of apoptosis

NCBI Summary:This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:O95865
NCBI GenInfo Identifier:6831529
NCBI Gene ID:23564
NCBI Accession:O95865.1
UniProt Related Accession:O95865
Molecular Weight:~ 30kDa
NCBI Full Name:N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
NCBI Synonym Full Names:dimethylarginine dimethylaminohydrolase 2
NCBI Official Symbol:DDAH2  
NCBI Official Synonym Symbols:G6a; DDAH; NG30; DDAHII; HEL-S-277  
NCBI Protein Information:N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
UniProt Protein Name:N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
UniProt Synonym Protein Names:DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein
Protein Family:N(G),N(G)-dimethylarginine dimethylaminohydrolase
UniProt Gene Name:DDAH2  
UniProt Entry Name:DDAH2_HUMAN
Anti-DDAH2 Antibody (CAB6457)Western blot analysis of extracts of various cell lines, using DDAH2 antibody (CAB6457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-DDAH2 Antibody (CAB6457)Immunofluorescence analysis of U2OS cells using DDAH2 antibody (CAB6457). Blue: DAPI for nuclear staining.

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