Anti-DDB2 Antibody (CAB1848)

Antibody Name:	Anti-DDB2 Antibody
Antibody SKU:	CAB1848
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPK KRPE TQKT SEIV LRPR NKRS RSPL ELEP EAKK LCAK GSGP SRRC DSDC LWVG LAGP QILP PCRS IVRT LHQH KLGR ASWP SVQQ GLQQ SFLH TLDS YRIL QKAA PFDR RATS LAWH PTHP STVA VGSK GGDI MLWN FGIK DKPT FIKG IGAG GSIT GLKF NPLN TNQF YASS MEGT TRLQ DFKG NILR VFAS SDTI NIWF CSLD VSAS SRMV VTGD NVGN VILL NMDG KELW NLRM HKKK VTHV ALNP CCDW FLAT ASVD QTVK IWDL RQVR GKAS FLYS LPHR HPVN AACF SPDG ARLL TTDQ KSEI RVY
Gene ID:	1643
Uniprot:	Q92466
Cellular Location:	Nucleus
Calculated MW:	17kDa/26kDa/40kDa/47kDa
Observed MW:	48kDa

Synonyms:

DDB2, DDBB, UV-DDB2, XPE

Background:

This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	DDB2: Required for DNA repair. Binds to DDB1 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4- ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair. Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4- ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1), which includes CUL4A or CUL4B, DDB1, DDB2 and RBX1. DDB2 may function as the substrate recognition module within this complex. The DDB1- CUL4-ROC1 complex may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. A large number of other DCX complexes may also exist in which an alternate substrate targeting subunit replaces DDB2. These targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Isoform D1 and isoform D2 do not interact with DDB1. Expression is induced in response to treatment with IR or UV and this requires p53/TP53 activity. Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. Belongs to the WD repeat DDB2/WDR76 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 11p12-p11 Cellular Component: nucleoplasm; protein complex; cell junction Molecular Function:protein binding; DNA binding; damaged DNA binding; ubiquitin-protein ligase activity Biological Process: protein autoubiquitination; protein polyubiquitination; nucleotide-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA damage removal; DNA repair; response to UV Disease: Xeroderma Pigmentosum, Complementation Group E
NCBI Summary:	This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:	Q92466
NCBI GenInfo Identifier:	12230033
NCBI Gene ID:	1643
NCBI Accession:	Q92466.1
UniProt Secondary Accession:	Q92466,Q76E54, Q76E55, Q76E56, Q76E57, B2R875,
UniProt Related Accession:	Q92466
Molecular Weight:	427
NCBI Full Name:	DNA damage-binding protein 2
NCBI Synonym Full Names:	damage-specific DNA binding protein 2, 48kDa
NCBI Official Symbol:	DDB2
NCBI Official Synonym Symbols:	DDBB; UV-DDB2
NCBI Protein Information:	DNA damage-binding protein 2; UV-DDB 2; DDB p48 subunit; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; xeroderma pigmentosum group E protein
UniProt Protein Name:	DNA damage-binding protein 2
UniProt Synonym Protein Names:	DDB p48 subunit; DDBb; Damage-specific DNA-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2
Protein Family:	DNA damage-binding protein
UniProt Gene Name:	DDB2
UniProt Entry Name:	DDB2_HUMAN

	Western blot analysis of extracts of various cell lines, using DDB2 antibody (CAB1848) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using DDB2 antibody (CAB1848). Blue: DAPI for nuclear staining.

Antibodies

Anti-DDB2 Antibody (CAB11615)

DDB2 Antibody (PACO08775)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)