Anti-DDX21 Antibody (CAB7034)

Antibody Name:	Anti-DDX21 Antibody
Antibody SKU:	CAB7034
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 624-783 of human DDX21 (NP_004719.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, MCF7, HepG2, 293T, Mouse spleen, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 624-783 of human DDX21 (NP_004719.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VDQR SLIN SNVG FVTM ILQC SIEM PNIS YAWK ELKE QLGE EIDS KVKG MVFL KGKL GVCF DVPT ASVT EIQE KWHD SRRW QLSV ATEQ PELE GPRE GYGG FRGQ REGS RGFR GQRD GNRR FRGQ REGS RGPR GQRS GGGN KSNR SQNK GQKR SFSK AFGQ
Gene ID:	9188
Uniprot:	Q9NR30
Cellular Location:	Nucleus, nucleolus, nucleoplasm
Calculated MW:	79kDa/87kDa
Observed MW:	100kDa

Synonyms:

DDX21, GUA, GURDB, RH-II/GU, RH-II/GuA

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.

UniProt Protein Function:	DDX21: a protein of the DEAD box helicase family. Unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
UniProt Protein Details:	Protein type:EC 3.6.4.13; RNA processing; Helicase; Nucleolus; RNA-binding Chromosomal Location of Human Ortholog: 10q21 Cellular Component: membrane; nucleolus; nucleus Molecular Function:double-stranded RNA binding; ATP-dependent RNA helicase activity; ATP binding Biological Process: osteoblast differentiation; response to exogenous dsRNA; metabolic process; response to virus
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NR30
NCBI GenInfo Identifier:	76803555
NCBI Gene ID:	9188
NCBI Accession:	Q9NR30.5
UniProt Secondary Accession:	Q9NR30,Q13436, Q5VX41, Q68D35, B2RDL0,
UniProt Related Accession:	Q9NR30
Molecular Weight:	783
NCBI Full Name:	Nucleolar RNA helicase 2
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box helicase 21
NCBI Official Symbol:	DDX21
NCBI Official Synonym Symbols:	GUA; GURDB; RH-II/GU; RH-II/GuA
NCBI Protein Information:	nucleolar RNA helicase 2; RH II/Gu; gu-alpha; Gu protein; DEAD box protein 21; RNA helicase II/Gu alpha; nucleolar RNA helicase Gu; nucleolar RNA helicase II; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
UniProt Protein Name:	Nucleolar RNA helicase 2
UniProt Synonym Protein Names:	DEAD box protein 21; Gu-alpha; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu
Protein Family:	Nucleolar RNA helicase
UniProt Gene Name:	DDX21
UniProt Entry Name:	DDX21_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX21 antibody (CAB7034) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using DDX21 antibody (CAB7034) at dilution of 1:100 (40x lens).

Antibodies

Anti-DDX21 Antibody (CAB14820)

Anti-DDX21 Antibody (CAB6017)

DDX21 Antibody (PACO08785)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)