The Delta-sarcoglycan (SGCD) Monoclonal Antibody (CAB9175) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, offers high reactivity with human samples and is validated for use in various applications, including Western blotting.Delta-sarcoglycan is a critical component of the dystrophin-glycoprotein complex, essential for maintaining muscle integrity and function. Dysregulation of delta-sarcoglycan has been linked to muscular dystrophy and other muscle-related disorders, making it a key target for research in the field of muscle biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Delta-sarcoglycan (SGCD) Monoclonal Antibody
SKU:
CAB9175
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1463
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Cytoplasm, Single-Pass Type Ii Membrane Protein, Cytoskeleton, Sarcolemma.
Calculated MW:
32kDa
Observed MW:
36kDa
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
6444
RRID
AB_2863679
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Delta-sarcoglycan (SGCD) Rabbit mAb (CAB9175) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from Mouse heart, using Delta-sarcoglycan (SGCD) Rabbit mAb (CAB9175) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
