The DHFR Monoclonal Antibody (CAB9299) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, is highly specific and sensitive for detecting DHFR in a variety of samples, including human tissues. It has been validated for use in Western blot applications, enabling precise and accurate analysis of DHFR expression levels.DHFR, also known as dihydrofolate reductase, is essential for the synthesis of nucleotides and DNA replication. It is a critical target for cancer chemotherapy, as inhibiting DHFR can disrupt cancer cell growth and proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DHFR Monoclonal Antibody
SKU:
CAB9299
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1513
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DYR, DHFR1, DHFRP1, DHFR
Positive Sample:
293T, Hep G2, C2C12, Mouse liver, Rat kidney
Cellular Localization:
Mitochondrion, Cytoplasm .
Calculated MW:
21kDa
Observed MW:
21kDa
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1719
RRID
AB_2863706
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DHFR Rabbit mAb (CAB9299) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
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