Anti-DHX36 Antibody (CAB5191)

Antibody Name:	Anti-DHX36 Antibody
Antibody SKU:	CAB5191
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DHX36 (NP_065916.2).

Application:	WB IP
Recommended Dilution:	WB 1:200 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse liver, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DHX36 (NP_065916.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSYD YHQN WGRD GGPR SSGG GYGG GPAG GHGG NRGS GGGG GGGG GGRG GRGR HPGH LKGR EIGM WYAK KQGQ KNKE AERQ ERAV VHMD ERRE EQIV QLLN SVQA KNDK ESEA QISW FAPE DHGY GTEV STKN TPCS ENKL DIQE KKLI NQEK KMFR IRNR SYID RDSE YLLQ ENEP DGTL DQKL LEDL QKKK NDLR YIEM QHFR EKLP SYGM QKEL VNLI DNHQ VTVI SGET GCGK TTQV TQFI LDNY IERG KGSA CRIV
Gene ID:	170506
Uniprot:	Q9H2U1
Cellular Location:	Chromosome, Cytoplasm, Nucleus, telomere
Calculated MW:	111kDa/113kDa/114kDa
Observed MW:	114kDa

Synonyms:

DHX36, DDX36, G4R1, MLEL1, RHAU

Background:

This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	DHX36: Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.4.13; EC 3.6.4.12; Helicase; RNA-binding Chromosomal Location of Human Ortholog: 3q25.2 Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; nucleus Molecular Function:G-quadruplex DNA binding; DNA-dependent ATPase activity; protein binding; histone deacetylase binding; double-stranded RNA binding; ATP-dependent RNA helicase activity; ATP binding Biological Process: RNA processing; ossification; response to exogenous dsRNA; transcription, DNA-dependent; response to virus; RNA secondary structure unwinding; positive regulation of telomere maintenance; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H2U1
NCBI GenInfo Identifier:	313104099
NCBI Gene ID:	170506
NCBI Accession:	Q9H2U1.2
UniProt Secondary Accession:	Q9H2U1,Q70JU3, Q8IYE5, Q9P240, B2RB00,
UniProt Related Accession:	Q9H2U1
Molecular Weight:	1008
NCBI Full Name:	ATP-dependent RNA helicase DHX36
NCBI Synonym Full Names:	DEAH (Asp-Glu-Ala-His) box polypeptide 36
NCBI Official Symbol:	DHX36
NCBI Official Synonym Symbols:	G4R1; RHAU; DDX36; MLEL1
NCBI Protein Information:	ATP-dependent RNA helicase DHX36; G4 resolvase-1; G4-resolvase 1; MLE-like protein 1; DEAH box protein 36; probable ATP-dependent RNA helicase DHX36; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36; RNA helicase associated with AU-rich element ARE
UniProt Protein Name:	ATP-dependent RNA helicase DHX36
UniProt Synonym Protein Names:	DEAH box protein 36; G4-resolvase 1; G4R1; MLE-like protein 1; RNA helicase associated with AU-rich element ARE
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DHX36
UniProt Entry Name:	DHX36_HUMAN

	Western blot analysis of extracts of various cell lines, using DHX36 antibody (CAB5191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug DHX36 antibody (CAB5191). Western blot was performed from the immunoprecipitate using DHX36 antibody (CAB5191) at a dilition of 1:1000.

Antibodies

DHX36 Antibody (PACO08833)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)