The DKK3 Antibody (CAB2527) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody was generated in rabbits and is specifically designed to react with human samples, making it ideal for use in Western blot applications.The DKK3 protein is a member of the Dickkopf family, known for its role in the Wnt signaling pathway, which is crucial for cell development and tissue homeostasis. Dysregulation of DKK3 has been implicated in diseases such as cancer, cardiovascular disorders, and neurological conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DKK3 Antibody
SKU:
CAB2527
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIG, CRRL, REIC, DKK3
Positive Sample:
293T, A-549, SH-SY5Y, Mouse brain, Mouse eye
Cellular Localization:
Secreted.
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
27122
RRID
AB_2764418
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DKK3 Rabbit pAb (CAB2527) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of MCF7 cells using DKK3 Rabbit pAb (CAB2527) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
