Description
dnaA Recombinant Protein (N-His)
dnaA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ile437, with an N-terminal His tag. Predicted molecular weight: 52.92 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Chromosomal replication initiator protein DnaA is a ~50 kDa protein. Plays an essential role in the initiation and regulation of chromosomal replication. For research use only.
ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase.
| Product Name: | dnaA Recombinant Protein (N-His) |
| SKU: | GXRP09968 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mycoplasma genitalium |
| Synonyms: | Chromosomal replication initiator protein DnaA; dnaA; MG469 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ile437 |
| Calculated MW: | 52.92 kDa |
| UniProt: | P35888 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
