Description
dNTP mix for PCR
dNTP Mix (10 mM each) is a ready-to-use, equimolar mixture of the four deoxynucleotides (dATP, dCTP, dGTP and dTTP) at 10 mM each. It is supplied at high purity for PCR, qPCR, reverse transcription and other enzymatic reactions, and is quality-tested for consistent amplification.
Purity is >99% by HPLC, and the mix is free from PCR, qPCR and reverse transcription inhibitors. It is a sodium salt solution (pH 7.0–9.0) that can be used directly without dilution in PCR, reverse transcription (RT) and one-step RT-PCR. For research use only.
Key Features
| Ready to use | Premixed aqueous dATP, dCTP, dGTP and dTTP at 10 mM each, used without dilution. | |
| High purity | >99% purity confirmed by HPLC. | |
| Inhibitor-free | Free from PCR, qPCR and reverse transcription inhibitors. | |
| Versatile | Suitable for PCR, reverse transcription and one-step RT-PCR. |
Specifications
| Composition | dATP, dCTP, dGTP and dTTP, 10 mM each (equimolar) |
| Formulation | Sodium salt solution, pH 7.0–9.0 |
| Purity | >99% (HPLC) |
| Applications | PCR, qPCR, reverse transcription (RT), one-step RT-PCR |
| Recommended use | 1 μl per 50 μl PCR; 1 μl per 20 μl RT reaction |
| Shelf life | 2 years |
Components
| Components | Amount |
| dNTP Mix (10 mM each) | 1 ml / 5 × 1 ml |
Supplied as a sodium salt solution of dNTPs, pH 7.0–9.0.
Storage
Store at -30 ~ -15°C.
- Use the mix directly, without dilution.
- For a 50 μl PCR, add 1 μl of dNTP Mix (10 mM each).
- For a 20 μl reverse transcription reaction, add 1 μl of dNTP Mix (10 mM each).
Q1: How much dNTP Mix should I add to a reaction?
- Add 1 μl to a 50 μl PCR or to a 20 μl reverse transcription reaction. The mix is used directly without dilution.
Q2: What is the purity and formulation?
- Each dNTP is >99% pure by HPLC. The mix is an equimolar sodium salt solution of dATP, dCTP, dGTP and dTTP at 10 mM each, pH 7.0–9.0, free from PCR, qPCR and RT inhibitors.
