Anti-DPP3 Antibody (CAB7054)

Antibody Name:	Anti-DPP3 Antibody
Antibody SKU:	CAB7054
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DPP3 (NP_569710.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HT-29, Jurkat, SKOV3, MCF7, Mouse liver, Mouse thymus, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DPP3 (NP_569710.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADT QYIL PNDI GVSS LDCR EAFR LLSP TERL YAYH LSRA AWYG GLAV LLQT SPEA PYIY ALLS RLFR AQDP DQLR QHAL AEGL TEEE YQAF LVYA AGVY SNMG NYKS FGDT KFVP NLPK EKLE RVIL GSEA AQQH PEEV RGLW QTCG ELMF SLEP RLRH LGLG KEGI TTYF SGNC TMED AKLA QDFL DSQN LSAY NTRL FKEV DGEG KPYY EVRL ASVL GSEP SLDS EVTS KLKS YEFR GSPF QVTR GDYA PILQ KVVE QLEK AKAY AANS HQGQ MLAQ
Gene ID:	10072
Uniprot:	Q9NY33
Cellular Location:	Cytoplasm
Calculated MW:	35kDa/69kDa/79kDa/82kDa
Observed MW:	83kDa

Synonyms:

DPP3, DPPIII

Background:

This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	DPP3: Cleaves Arg-Arg-beta-naphthylamide. Belongs to the peptidase M49 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.14.4; Protease Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: nucleoplasm; cytoplasm; plasma membrane Molecular Function:protein binding; zinc ion binding; metallopeptidase activity; dipeptidyl-peptidase activity Biological Process: proteolysis
NCBI Summary:	This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q9NY33
NCBI GenInfo Identifier:	20532389
NCBI Gene ID:	10072
NCBI Accession:	Q9NY33.2
UniProt Secondary Accession:	Q9NY33,O95748, Q969H2, Q9BV67, Q9HAL6, B2RDB5, B4DLX4 F5H8L6,
UniProt Related Accession:	Q9NY33
Molecular Weight:	737
NCBI Full Name:	Dipeptidyl peptidase 3
NCBI Synonym Full Names:	dipeptidyl-peptidase 3
NCBI Official Symbol:	DPP3
NCBI Official Synonym Symbols:	DPPIII
NCBI Protein Information:	dipeptidyl peptidase 3; DPP III; enkephalinase B; dipeptidyl peptidase III; dipeptidyl arylamidase III; dipeptidyl aminopeptidase III
UniProt Protein Name:	Dipeptidyl peptidase 3
UniProt Synonym Protein Names:	Dipeptidyl aminopeptidase III; Dipeptidyl arylamidase III; Dipeptidyl peptidase III; DPP III; Enkephalinase B
UniProt Gene Name:	DPP3
UniProt Entry Name:	DPP3_HUMAN

	Western blot analysis of extracts of various cell lines, using DPP3 antibody (CAB7054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spinal cord using DPP3 Antibody (CAB7054) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using DPP3 Antibody (CAB7054) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-DPP3 Antibody (CAB13390)	Human DPP3 / Dipeptidyl peptidase 3 ELISA Kit
DPP3 Antibody (PACO08923)	Human Dipeptidyl peptidase 3 (DPP3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)