Description
dTTP solution for PCR
dTTP (100 mM) is a high-purity, single-nucleotide solution for PCR, sequencing, labelling and other molecular biology reactions that require individual deoxynucleotides.
Purity is >99% by HPLC, and the product is free from qPCR, PCR and reverse transcription inhibitors. Applications include PCR, real-time PCR, RT-PCR, cDNA or general DNA synthesis, primer extension, DNA sequencing and DNA labelling. For research use only.
Key Features
| Flexible PCR companion | A single component that lets you configure dNTP mixes at different concentrations. | |
| >99% purity | Purity confirmed by HPLC. | |
| Inhibitor-free | Free from qPCR, PCR and reverse transcription inhibitors. | |
| Broad application | Suitable for PCR, qPCR, RT-PCR, DNA synthesis, primer extension, sequencing and labelling. |
Specifications
| Concentration | 100 mM (100 mmol/L) |
| Formulation | Sodium salt solution, pH 7.0–9.0 |
| Purity | >99% (HPLC) |
| Applications | PCR, real-time PCR, RT-PCR, cDNA or DNA synthesis, primer extension, DNA sequencing, DNA labelling |
| Shelf life | 2 years |
Components
| Components | Amount |
| dTTP (100 mM) | 1 ml |
Storage
Store at -30 ~ -15°C.
- Prepare the dNTPs
- Combine with other individual dNTPs, or prepare separately, at the concentrations your amplification requires.
- Add to the reaction
- 50 μl standard PCR: use 0.1 μl.
- 20 μl standard RT reaction: use 0.1 μl.
Q1: How much dTTP should I add to a reaction?
- The recommended amount is 0.1 μl for a 50 μl standard PCR and 0.1 μl for a 20 μl standard RT reaction.
Q2: What is the formulation of the product?
- A 100 mM sodium salt solution at pH 7.0–9.0, with >99% purity by HPLC.
Q3: How should I use dTTP alongside dUTP?
- When dUTP is used in PCR, use it at 1–3 times the concentration of dTTP. Increasing the MgCl2 concentration slightly can give better amplification.
