null

Anti-DAG1 Antibody (CAB10076)

SKU:
CAB10076
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-DAG1 Antibody CAB10076
  • Immunology Antibodies 1 Anti-DAG1 Antibody CAB10076
  • Immunology Antibodies 1 Anti-DAG1 Antibody CAB10076
  • Immunology Antibodies 1 Anti-DAG1 Antibody CAB10076
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-DAG1 Antibody
Antibody SKU:CAB10076
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:20 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, U2OS, SW480, HeLa, mouse skeletal muscle, mouse lung, mouse heart, rat liver, rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SIVV EWTN NTLP LEPC PKEQ IAGL SRRI AEDD GKPR PAFS NALE PDFK ATSI TVTG SGSC RHLQ FIPV VPPR RVPS EAPP TEVP DRDP EKSS EDDV
Gene ID:1605
Uniprot:Q14118
Cellular Location:Cell junction, Cell membrane, Cytoplasm, Nucleus, Secreted, Single-pass type I membrane protein, cytoskeleton, extracellular space, nucleoplasm, postsynaptic cell membrane, sarcolemma, synapse
Calculated MW:97kDa
Observed MW:43kDa/95kDa
Synonyms:156DAG, A3a, AGRNR, DAG, MDDGA9, MDDGC7, MDDGC9, DAG1
Background:This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
UniProt Protein Function:DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: dystroglycan complex; extracellular space; costamere; focal adhesion; basolateral plasma membrane; integral to membrane; extracellular region; lipid raft; nucleoplasm; dystrophin-associated glycoprotein complex; postsynaptic membrane; cytoskeleton; cell-cell adherens junction; lamellipodium; cytoplasm; plasma membrane; basement membrane; sarcolemma; filopodium

Molecular Function:tubulin binding; viral receptor activity; laminin-1 binding; protein binding; structural constituent of muscle; protein complex binding; SH2 domain binding; calcium ion binding; alpha-actinin binding; actin binding; vinculin binding

Biological Process: nerve maturation; response to peptide hormone stimulus; entry of virus into host cell; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; cytoskeletal anchoring; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; NLS-bearing substrate import into nucleus; myelination in the peripheral nervous system; virus-host interaction; morphogenesis of an epithelial sheet; calcium-dependent cell-matrix adhesion; negative regulation of cell migration

Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9

NCBI Summary:Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]
UniProt Code:Q14118
NCBI GenInfo Identifier:229462879
NCBI Gene ID:1605
NCBI Accession:Q14118.2
UniProt Secondary Accession:Q14118,Q969J9, A8K6M7,
UniProt Related Accession:Q14118
Molecular Weight:97,441 Da
NCBI Full Name:Dystroglycan
NCBI Synonym Full Names:dystroglycan 1 (dystrophin-associated glycoprotein 1)
NCBI Official Symbol:DAG1  
NCBI Official Synonym Symbols:A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9  
NCBI Protein Information:dystroglycan
UniProt Protein Name:Dystroglycan
UniProt Synonym Protein Names:Dystrophin-associated glycoprotein 1
Protein Family:Dystroglycan
UniProt Gene Name:DAG1  
UniProt Entry Name:DAG1_HUMAN
Anti-DAG1 Antibody (CAB10076)Western blot analysis of extracts of various cell lines, using DAG1 antibody (CAB10076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-DAG1 Antibody (CAB10076)Immunofluorescence analysis of H9C2 cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-DAG1 Antibody (CAB10076)Immunofluorescence analysis of L929 cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-DAG1 Antibody (CAB10076)Immunofluorescence analysis of U-2 OS cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Related Products