Description
E. coli pcnB/Poly(A) polymerase I Recombinant Protein (C-Strep)
E. coli pcnB/Poly(A) polymerase I Recombinant Protein (C-Strep) is a recombinant protein expressed in E. coli, spanning Leu13-Ala465, with a C-terminal Strep tag. Predicted molecular weight: 66.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Poly(A) polymerase I (pcnB) is a ~53 kDa protein. Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. For research use only.
Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.
| Product Name: | E. coli pcnB/Poly(A) polymerase I Recombinant Protein (C-Strep) |
| SKU: | GXRP06255 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Escherichia coli |
| Synonyms: | Poly(A) polymerase I, PAP I, 2.7.7.19, Plasmid copy number protein, pcnB, b0143, JW5808 |
| Tag: | C-Strep |
| Expression Host: | E. coli |
| Protein Length: | Leu13-Ala465 |
| Calculated MW: | 66.31 kDa |
| UniProt: | P0ABF1 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
