Anti-SELE Antibody (CAB2191)

Antibody Name:	Anti-SELE Antibody
Antibody SKU:	CAB2191
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, HeLa, Raji, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WSYN TSTE AMTY DEAS AYCQ QRYT HLVA IQNK EEIE YLNS ILSY SPSY YWIG IRKV NNVW VWVG TQKP LTEE AKNW APGE PNNR QKDE DCVE IYIK REKD VGMW NDER CSKK KLAL CYTA ACTN TSCS GHGE CVET INNY TCKC DPGF SGLK CEQI VNCT ALES PEHG SLVC SHPL GNFS YNSS CSIS CDRG YLPS SMET MQCM SSGE W
Gene ID:	6401
Uniprot:	P16581
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	66kDa
Observed MW:	70kDa

Synonyms:

SELE, CD62E, ELAM, ELAM1, ESEL, LECAM2

Background:

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.

UniProt Protein Function:	SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q22-q25 Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; plasma membrane; integral to membrane; coated pit; caveola; lipid raft Molecular Function:protein binding; transmembrane receptor activity; phospholipase binding; sialic acid binding Biological Process: positive regulation of leukocyte migration; positive regulation of receptor internalization; actin filament-based process; calcium-mediated signaling; response to lipopolysaccharide; leukocyte migration during inflammatory response; heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; regulation of inflammatory response; blood coagulation; inflammatory response; leukocyte tethering or rolling; leukocyte migration Disease: Hypertension, Essential
NCBI Summary:	The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]
UniProt Code:	P16581
NCBI GenInfo Identifier:	126180
NCBI Gene ID:	6401
NCBI Accession:	P16581.1
UniProt Secondary Accession:	P16581,P16111, A2RRD6,
UniProt Related Accession:	P16581
Molecular Weight:	66,655 Da
NCBI Full Name:	E-selectin
NCBI Synonym Full Names:	selectin E
NCBI Official Symbol:	SELE
NCBI Official Synonym Symbols:	ELAM; ESEL; CD62E; ELAM1; LECAM2
NCBI Protein Information:	E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
UniProt Protein Name:	E-selectin
UniProt Synonym Protein Names:	CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD_antigen: CD62E
Protein Family:	E-selectin
UniProt Gene Name:	SELE
UniProt Entry Name:	LYAM2_HUMAN

	Western blot analysis of extracts of various cell lines, using SELE antibody (CAB2191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse kidney using SELE Rabbit pAb (CAB2191) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using SELE Rabbit pAb (CAB2191) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using SELE Rabbit pAb (CAB2191) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
SELE Antibody (PACO07253)	Human E-selectin (SELE) ELISA Kit
SELE Antibody (PACO12091)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)