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Anti-EEF1E1 Antibody (CAB15357)

SKU:
CAB15357
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 1 Anti-EEF1E1 Antibody CAB15357
  • Metabolism Antibodies 1 Anti-EEF1E1 Antibody CAB15357
€139 - €419
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Description

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Antibody Name:Anti-EEF1E1 Antibody
Antibody SKU:CAB15357
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA AELS LLEK SLGL SKGN KYSA QGER QIPV LQTN NGPS LTGL TTIA AHLV KQAN KEYL LGST AEEK AIVQ QWLE YRVT QVDG HSSK NDIH TLLK DLNS
Gene ID:9521
Uniprot:O43324
Cellular Location:Cytoplasm, Nucleus
Calculated MW:15kDa/19kDa
Observed MW:20kDa
Synonyms:EEF1E1, AIMP3, P18
Background:This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.
UniProt Protein Function:EEF1E1: Positive modulator of ATM response to DNA damage.
UniProt Protein Details:

Protein type:Translation; Translation elongation

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function:protein binding

Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of apoptosis; gene expression; positive regulation of DNA damage response, signal transduction by p53 class mediator

NCBI Summary:This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]
UniProt Code:O43324
NCBI GenInfo Identifier:17378996
NCBI Gene ID:9521
NCBI Accession:O43324.1
UniProt Related Accession:O43324
Molecular Weight:20; 16; 20kDa
NCBI Full Name:Eukaryotic translation elongation factor 1 epsilon-1
NCBI Synonym Full Names:eukaryotic translation elongation factor 1 epsilon 1
NCBI Official Symbol:EEF1E1  
NCBI Official Synonym Symbols:P18; AIMP3  
NCBI Protein Information:eukaryotic translation elongation factor 1 epsilon-1
UniProt Protein Name:Eukaryotic translation elongation factor 1 epsilon-1
UniProt Synonym Protein Names:Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18
Protein Family:Eukaryotic translation elongation factor
UniProt Gene Name:EEF1E1  
UniProt Entry Name:MCA3_HUMAN
Anti-EEF1E1 Antibody (CAB15357)Western blot analysis of extracts of various cell lines, using EEF1E1 antibody (CAB15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-EEF1E1 Antibody (CAB15357)Immunofluorescence analysis of L929 cells using EEF1E1 antibody (CAB15357) at dilution of 1:100. Blue: DAPI for nuclear staining.

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