The EGF Antibody (CAB13615) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the EGF protein, this antibody allows for precise detection and analysis of EGF expression in a variety of cell types.EGF is a crucial growth factor that plays a key role in regulating cell proliferation, differentiation, and survival. Dysregulation of the EGF signaling pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EGF Antibody
SKU:
CAB13615
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
URG, HOMG4, EGF
Positive Sample:
Rat skeletal muscle
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
134kDa
This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Purification Method
Affinity purification
Gene ID
1950
RRID
AB_2760476
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat skeletal muscle, using EGF Rabbit pAb (CAB13615) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunofluorescence analysis of paraffin-embedded human skin using EGF Rabbit pAb (CAB13615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
