Anti-EGF Antibody (CAB2720)

Antibody Name:	Anti-EGF Antibody
Antibody SKU:	CAB2720
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human EGF

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney, 293T

Immunogen:	Recombinant protein of human EGF
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1950
Uniprot:	P01133
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	129kDa/133kDa
Observed MW:	160kDa

Synonyms:

EGF, HOMG4, URG

Background:

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

NCBI Summary:	This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:	P01133
NCBI GenInfo Identifier:	251757262
NCBI Gene ID:	1950
NCBI Accession:	P01133.2
UniProt Related Accession:	P01133
Molecular Weight:	6.2
NCBI Full Name:	Pro-epidermal growth factor
NCBI Synonym Full Names:	epidermal growth factor
NCBI Official Symbol:	EGF
NCBI Official Synonym Symbols:	URG; HOMG4
NCBI Protein Information:	pro-epidermal growth factor
UniProt Protein Name:	Pro-epidermal growth factor
UniProt Synonym Protein Names:	Urogastrone
Protein Family:	Epidermal growth factor
UniProt Gene Name:	EGF
UniProt Entry Name:	EGF_HUMAN

	Western blot analysis of extracts of mouse kidney, using EGF antibody (CAB2720) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of 293T cells, using EGF antibody (CAB2720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies	ELISA Kits
Anti-EGF Antibody (CAB12916)	Human EGF ELISA Kit
Anti-EGF Antibody (CAB13615)	Human EGF (Epidermal Growth Factor) CLIA Kit (HUES00057)
EGF Antibody (PACO02618)	Human EGF (Epidermal Growth Factor) ELISA Kit (HUES01346)
	Human Pro-epidermal growth factor (EGF) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)