The EIF2AK1 Antibody (CAB14119) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to EIF2AK1, researchers can easily detect and analyze this important protein in various cell types.EIF2AK1, also known as PERK, plays a key role in the unfolded protein response pathway, which helps cells cope with endoplasmic reticulum stress. Dysregulation of this pathway has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
EIF2AK1 Antibody
SKU:
CAB14119
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCR, HRI, hHRI, LEMSPAD, EIF2AK1
Positive Sample:
K-562, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
71kDa
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
27102
RRID
AB_2760975
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using EIF2AK1 Rabbit pAb (CAB14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Rat liver, using EIF2AK1 Rabbit pAb (CAB14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
