Western blot analysis of extracts from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using EIF2AK1 Rabbit mAb (CAB24614) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] EIF2AK1 Monoclonal Antibody (CAB24614) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal techniques, shows high specificity for EIF2AK1 and is validated for use in various experimental techniques, including Western blot and immunofluorescence.EIF2AK1, also known as eukaryotic translation initiation factor 2-alpha kinase 1, is a crucial component of the cellular stress response pathway, regulating protein synthesis in times of environmental stress.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] EIF2AK1 Monoclonal Antibody
SKU:
CAB24614
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC62929
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCR, HRI, hHRI, LEMSPAD, [KD Validated] EIF2AK1
Positive Sample:
293T(KD)
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
71kDa
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
27102
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb (CAB24614) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
