Western blot analysis of THP-1, using Bid Rabbit mAb (CAB23234) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] Bid Monoclonal Antibody (CAB23234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, validated for use in various applications including Western blotting and immunofluorescence, specifically binds to the BID protein, allowing for accurate detection and analysis in different cell types.BID, a pro-apoptotic member of the Bcl-2 protein family, is essential for the activation of the intrinsic apoptotic pathway in response to various cellular stresses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] Bid Monoclonal Antibody
SKU:
CAB23234
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59702
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.
Purification Method
Affinity purification
Gene ID
637
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using [KD Validated] Bid Rabbit mAb (CAB23234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from wild type(WT) and Bid knockdown (KD) 293T cells, using [KD Validated] Bid Rabbit mAb (CAB23234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
