The GCN2 Antibody (CAB7155) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits high specificity and sensitivity to human samples, making it ideal for use in Western blot applications. By binding to the EIF2AK4 protein, this antibody enables precise detection and analysis in various cell types, facilitating research in immunology, oncology, and inflammatory diseases.EIF2AK4, also known as GCN2, is a crucial player in the integrated stress response pathway, regulating protein synthesis in response to nutrient deprivation and cellular stress.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GCN2 Antibody
SKU:
CAB7155
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GCN2, PVOD2
Positive Sample:
HeLa, 293T, Mouse liver, Mouse brain, Rat liver, Rat brain
Cellular Localization:
Cytosol, Cytosolic Ribosome.
Calculated MW:
187kDa
Observed MW:
220kDa
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Purification Method
Affinity purification
Gene ID
440275
RRID
AB_2767709
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GCN2 Rabbit pAb (CAB7155) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of PC-12 cells using GCN2 Rabbit pAb (CAB7155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
