Anti-EIF3D Antibody (CAB5947)

Antibody Name:	Anti-EIF3D Antibody
Antibody SKU:	CAB5947
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SGC-7901, U-87MG, HeLa, NIH/3T3, Mouse liver, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKF MTPV IQDN PSGW GPCA VPEQ FRDM PYQP FSKG DRLG KVAD WTGA TYQD KRYT NKYS SQFG GGSQ YAYF HEED ESSF QLVD TART QKTA YQRN RMRF AQRN LRRD KDRR NMLQ FNLQ ILPK SAKQ KERE RIRL QKKF QKQF GVRQ KWDQ KSQK PRDS SVEV RSDW EVKE EMDF PQLM KMRY LEVS EPQD IECC GALE YYDK AFDR ITTR SEKP LRSI KRIF HTVT TTDD PVIR KLAK
Gene ID:	8664
Uniprot:	O15371
Cellular Location:	Cytoplasm
Calculated MW:	58kDa/62kDa/63kDa
Observed MW:	64kDa

Synonyms:

EIF3D, EIF3S7, eIF3-p66, eIF3-zeta

Background:

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.

UniProt Protein Function:	eIF3-zeta: eukaryotic translation initiation factor 3 subunit 7. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the subunit p170 of eIF-3. eIF-3 is composed of at least 12 different subunits.
UniProt Protein Details:	Protein type:Translation; Translation initiation; RNA-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex; membrane Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; formation of translation initiation complex; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation
NCBI Summary:	Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]
UniProt Code:	O15371
NCBI GenInfo Identifier:	6685526
NCBI Gene ID:	8664
NCBI Accession:	O15371.1
UniProt Secondary Accession:	O15371,Q3MJD9, Q5M9Q6, A8MWD3, B2R7D4, B4DTF8, B4DVY1
UniProt Related Accession:	O15371
Molecular Weight:	62,040 Da
NCBI Full Name:	Eukaryotic translation initiation factor 3 subunit D
NCBI Synonym Full Names:	eukaryotic translation initiation factor 3 subunit D
NCBI Official Symbol:	EIF3D
NCBI Official Synonym Symbols:	EIF3S7; eIF3-p66; eIF3-zeta
NCBI Protein Information:	eukaryotic translation initiation factor 3 subunit D
UniProt Protein Name:	Eukaryotic translation initiation factor 3 subunit D
UniProt Synonym Protein Names:	Eukaryotic translation initiation factor 3 subunit 7
UniProt Gene Name:	EIF3D
UniProt Entry Name:	EIF3D_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF3D antibody (CAB5947) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L-929 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L-929 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

EIF3D Antibody (PACO05597)

EIF3D Antibody (PACO09044)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)